Pharmacological evaluation of anti-wrinkle creams containing estrogen vitamin C and E

Abstract

The skin is the largest organ in the body, accounting for around 15% of total body weight. The ageing process in humans is more evident in the skin in the form of fine lines and wrinkles. Aging can be brought on by a number of internal and external causes. Internal causes include the loss of estrogen hormone in postmenopausal women, which is one of the leading causes of skin wrinkle. To overcome above mentioned problems two topical creams were formulated, one containing estradiol and other one containing estradiol in combination with antioxidants i.e. vitamin C and E, which were evaluated physically. These were applied on the wrinkled skin of rats and their anti-wrinkle activity was observed. The skin of all three groups were examined for any reduction in wrinkles every three days for 15 days. At the day 0, there were about 15 to 20 wrinkles on 6 cm square are of rat’s skin on average. But when the creams were applied and wrinkle scoring was observed every 3 days for 15 days the results of the creams containing estrogen and that containing estrogen + vitamin C & E were very good showing gradual decrease in wrinkles from 12, 15 on average to 2, 3 and even zero at the end of 15 days while the control cream failed to remove wrinkles from rats skin. Also the cream containing estrogen along with vitamin C and E showed the smoothness of skin and removes the dry patches and marks from the rat’s skin. Efficacy of the preparations was determined by wrinkle scoring study of the skin of rats. The study showed the positive outcome of the experiments done on rat’s wrinkled skin. It showed that estrogen cream should be used topically for the reduction of wrinkles and to improve the skin elasticity in advanced age population especially in post-menopausal women.